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Thursday, November 26, 2020 | History

2 edition of Interactions of antimicrobial peptides with biofilms of Pseudomonas aeruginosa. found in the catalog.

Interactions of antimicrobial peptides with biofilms of Pseudomonas aeruginosa.

Celine Sze Lai Chan

Interactions of antimicrobial peptides with biofilms of Pseudomonas aeruginosa.

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Published .
Written in English


About the Edition

The chronicity of Pseudomonas aeruginosa infections in cystic fibrosis (CF) patients is characterized by its overproduction of the exopolysaccharide alginate, in which bacteria are embedded. Alginate contributes to biofilm-related resistance by acting as a diffusion barrier to positively-charged antimicrobial agents including cationic antimicrobial peptides. Our laboratory recently developed a new category of non-amphipathic antimicrobial peptides originally designed to be transmembrane mimetic model peptides. Peptides of this group above a specific hydrophobicity threshold insert spontaneously into membranes and have antibacterial activity at micromolar concentrations. While investigating the molecular basis of biofilm resistance to peptides, we found that the anionic alginate induces conformational changes in hydrophobic antimicrobial peptides typically associated with insertion of such peptides into membrane environments. The overall results indicate that hydrophilic alginate polymers contain a significant hydrophobic compartment, and behave as an "auxiliary membrane" for bacteria, thus identifying a unique protective role for biofilm matrices.

The Physical Object
Pagination133 leaves.
Number of Pages133
ID Numbers
Open LibraryOL19512274M
ISBN 100612951863

This work aims at characterizing endoscope biofilm-isolated (PAI) and reference strain P. aeruginosa (PA) adhesion, biofilm formation and sensitivity to antibiotics. The recovery ability of the biofilm-growing bacteria subjected to intermittent antibiotic pressure (ciprofloxacin (CIP) and gentamicin (GM)), as well as the development of resistance towards antibiotics and benzalkonium chloride.   More than 40 antimicrobial peptides and proteins (AMPs) are expressed in the oral cavity. Antimicrobial peptide GL13K is effective in reducing biofilms of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 57 Synergistic interactions of vancomycin with different antibiotics against escherichia coli.


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Interactions of antimicrobial peptides with biofilms of Pseudomonas aeruginosa. by Celine Sze Lai Chan Download PDF EPUB FB2

A Novel RNase 3/ECP Peptide for Pseudomonas aeruginosa Biofilm Eradication That Combines Antimicrobial, Lipopolysaccharide Binding, and Cell-Agglutinating Activities. Antimicrobial Agents and Chemotherapy60 (10), DOI: /AACCited by: We have now developed novel amino-acid anti-biofilm peptides that (i) kill multiple species of bacteria in biofilms (MBEC change to minimal biofilm eradication concentration aeruginosa and other major clinically relevant Gram negative and Gram positive bacteria, including the ESKAPE pathogens, (ii) work synergistically with antibiotics in multiple species, and (iii) are effective in animal models.

Pseudomonas aeruginosa (P. aeruginosa) biofilms are associated with a wide range of infections, from chronic tissue diseases to implanted medical devices. In a biofilm, the extracellular polymeric substance Interactions of antimicrobial peptides with biofilms of Pseudomonas aeruginosa. book causes an inhibited penetration of antibacterial agents, leading to a – times tolerance of the by: 1.

Pseudomonas aeruginosa PAO1‐GFP‐tagged biofilms were grown in center for disease control (CDC) biofilm reactors (Biosurface Technologies Inc., Bozeman, MT). This method is routinely used to study the formation of biofilms by P.

aeruginosa grown with high shear and continuous flow (Goeres et al. ).Cited by: The antibiotic resistance of Pseudomonas aeruginosa (P. aeruginosa) is correlated with the formation of biofilms. Several studies have focused on biofilms and the treatment of biofilm infection by antimicrobial peptides (AMPs).Author: Dengfeng Xu, Yang Zhang, Peng Cheng, Yidong Wang, Xiaofen Li, Zhiying Wang, Huashan Yi, Hongwei Chen.

In general, the specific physiology of biofilms and the barrier function of the extracellular biofilm matrix determine resistance to antibacterials. However, resistance to antimicrobial peptides appears to be mainly based on the interaction with biofilm and capsule exopolymers.

Anti-biofilm activity. Pseudomonas aeruginosa ATCC and S. aureus ATCC were cultured in MHB media. Bacteria were diluted in MHB media containing % glucose at 5 × 10 5 CFU/mL and the diluted bacteria and peptides were added to a well plate, which was incubated at 37 °C.

After 24 h, the supernatant was discarded, and biofilms. Pseudomonas aeruginosa is a major health challenge that causes recalcitrant multidrug-resistant infections, especially in immunocompromised and hospitalized patients.P.

aeruginosa is an important cause of nosocomial and ventilator-associated pneumonia characterized by high prevalence and fatality rates.P. aeruginosa also causes chronic lung infections in individuals with cystic fibrosis.

Pseudomonas aeruginosa colonization and biofilm formation is responsible for numerous infections, including chronic infections in cystic fibrosis lung, diabetic foot ulcers, burn wounds, urinary tract infections, eye infections, hospital acquired infections, which lead to morbidity and mortality.

Biofilm formation is triggered through Quorum sensing (QS) mechanism and via the QS mechanism. Alginate can interact with cationic AMPs and protect P. aeruginosa biofilm from the effect of the antimicrobial peptides. Moreover, the peptide sensing system known as aps, first recognized in S.

epidermidis, can protect Gram-positive bacteria from AMP action. This system upregulates the D-alanylation of teichoic acid and increases the. Chronic infections with the opportunistic pathogen Pseudomonas aeruginosa are responsible for the majority of the morbidity and mortality in patients with cystic fibrosis (CF).

While P. aeruginosa infections may initially be treated successfully with standard antibiotics, chronic infections typically arise as bacteria transition to a biofilm mode of growth and acquire remarkable antimicrobial. Pseudomonas aeruginosa and Staphylococcus aureus often cause chronic, recalcitrant infections in large part due to their ability to form biofilms.

The biofilm mode of growth enables these organisms to withstand antibacterial insults that would effectively eliminate their planktonic counterparts. We found that P. aeruginosa supernatant increased the sensitivity of S.

aureus biofilms to multiple. Extracellular DNA (eDNA) is in the environment, bodily fluids, in the matrix of biofilms, and accumulates at infection sites. eDNA can function as a nutrient source, a universal biofilm matrix component, and an innate immune effector in eDNA traps.

In biofilms, eDNA is required for attachment, aggregation, and stabilization of microcolonies. We have recently shown that eDNA can sequester.

Pseudomonas aeruginosa infection is a predominant cause of morbidity and mortality in patients with cystic fibrosis infection and with a compromised immune system. Emergence of bacterial resistance renders existing antibiotics inefficient, and therefore discovery of new antimicrobial agents is highly warranted.

In recent years, numerous studies have demonstrated that antimicrobial peptides. Pseudomonas aeruginosa is a gram-negative pathogen that has become an important cause of infection, especially in patients with compromised host defense mechanisms. It is frequently related to nosocomial infections such as pneumonia, urinary tract infections (UTIs) and bacteremia.

The biofilm. Now, in the study “ Activity of a novel antimicrobial peptide against Pseudomonas aeruginosa biofilms, ” published in the journal Scientific Reports, a team developed a synthetic CAP and showed that it has anti-biofilm activity.

CAPs are found in a variety of organisms, and constitute a major component of the innate immune system. Here we provide evidence that a spatially distinct subpopulation of metabolically active cells in Pseudomonas aeruginosa biofilms is able to develop tolerance to the antimicrobial peptide colistin.

On the contrary, biofilm cells exhibiting low metabolic activity were killed by colistin. We had previously reported a peptide sequence, Arg–Ser–Ser (RSS), which was identified after coincubation of an edible orchid species, Dendrobium aphyllum, with Lactobacillus this study, we investigated the potential mechanism of the antibacterial effect of RSS on the pathogen Pseudomonas minimum inhibitory concentration of RSS for P.

aeruginosa was. Pseudomonas aeruginosa is one of most fatal known patho-gens, causinghospital-acquired infections annually in the United States (17). More than 80% of cystic fibrosis (CF) patients are infected by P. aeruginosa (13, 26), and chronic CF infections result from the formation of P.

aeruginosabiofilms in the lung (14, 27). Keywords:Cationic antimicrobial peptides, alginate, biofilm, circular dichroism spectroscopy, Raman spectroscopy, peptide-exopolysaccharide complexes.

Abstract: Alginate is a biofilm exopolysaccharide secreted by the opportunistic pathogen Pseudomonas aeruginosa that acts to prevent the diffusion of antibiotics toward the bacterial cell membrane. Biofilms and planktonic cells of Pseudomonas aeruginosa have similar resistance to killing by antimicrobials.

Journal of Bacteriology,– Stewart, P. the obtained results. The novel antimicrobial agent tested was the anti-biofilm peptide DJK-5, which inhibits bio-film formation and eradicates preformed biofilms constructed from a wide range of microorganisms, including P. aeruginosa, on abiotic surfaces and invertebrate in vivo models DJK-5 is a D-enantiomeric cationic peptide that.

Biofilm matrices of two Klebsiella pneumoniae clinical isolates, KpTs and KpTs, were investigated for their polysaccharide composition and protective effects against antimicrobial peptides. Both strains were good biofilm producers, with KpTs forming flocs with very low adhesive properties to supports.

Matrix exopolysaccharides were isolated and their monosaccharide composition and. Anti-biofilm peptides may act by targeting an al Esin, S. Antimicrobial peptides and their interaction with biofilms of medically A novel RNase 3/ECP peptide for Pseudomonas aeruginosa.

The biofilm formation assay for P. aeruginosa was performed using the Calgary protocol as described previously. 16 The bacterial biofilm was formed by immersing the pegs of a modified polystyrene microtiter lid into a well microtiter plate containing μL of the overnight culture diluted in fresh LB medium (catalog no.

; Nunc. Pseudomonas aeruginosa is a ubiquitous environmental Gram-negative bacterium that is also a major opportunistic human pathogen in nosocomial infections and cystic fibrosis chronic lung infections.

These P. aeruginosa infections can be extremely difficult to treat due to the high intrinsic antibiotic resistance and broad repertoire of virulence factors, both of which are highly regulated.

Nagant C, Pitts B, Nazmi K, Vandenbranden M, Bolscher JG, Stewart PS, et al. Identification of peptides derived from the human antimicrobial peptide LL active against biofilms formed by Pseudomonas aeruginosa using a library of truncated fragments.

Antimicrob Agents Chemother. ;56(11)– pmid View Article. Cationic antimicrobial peptides (CAMPs) are very promising in the treatment of multi-drug resistant Pseudomonas aeruginosa lung infections experienced by cystic fibrosis (CF) patients.

Nevertheless, there is an urgent need of inhalable formulations able to deliver the intact CAMP in conductive airways and to shield its interactions with airway. The ability to form biofilms is a critical factor in chronic infections by Pseudomonas aeruginosa and has made this bacterium a model organism with respect to biofilm formation.

This study describes a new, previously unrecognized role for the human cationic host defense peptide LL In addition to its key role in modulating the innate immune response and weak antimicrobial activity, LL Search for predicted protein-protein interactions using peptides in Pseudomonas aeruginosa.

McPhee JB, Lewenza S, Hancock RE Mol Microbiol Oct;50(1) PubMed ID: Deficient Lipid A Remodeling by the arnB Gene Promotes Biofilm Formation in Antimicrobial Peptide Susceptible Pseudomonas aeruginosa. In an attempt to try to prevent the formation of biofilms or to break up existing biofilms of pathogenic bacteria, herein we have used the standard crystal violet assay as well as the Calgary biofilm device, to test several lactoferrin- and lactoferricin-derived antimicrobial peptides for their antibiofilm activity against Pseudomonas.

Circular dichroism and 1H NMR were used to investigate the interactions of a series of synthetic antimicrobial peptides (AMPs) with lipopolysaccharides (LPS) isolated from Pseudomonas aeruginosaand Klebsiella pneumoniae. Previous CD studies with AMPs containing only three Tic-Oic dipeptide units do not exhibit helical characteristics upon.

Pseudomonas aeruginosa is a pathogenic bacterium known to cause serious human infections, especially in immune‐compromised patients.

This is due to its unique ability to transform from a drug‐tolerant planktonic to a more dangerous and treatment‐resistant sessile life form, called biofilm. Abstract. The emergence of carbapenem-resistant Acinetobacter baumannii and Pseudomonas aeruginosa raises fears of untreatable infections and poses the greatest health threats.

Antimicrobial peptides (AMPs) are regarded as the most ideal solution to this menace. In this study, a set of peptides was designed based on our previously reported peptide cathelicidin-BF, and the lead peptide. to evaluate the antimicrobial efficacy of temporin A and the short lipopeptides (C10)2-KKKK-NH2 and (C12)2-KKKK-NH2 in combination with gentamicin against biofilm formed by Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA).

Peptides were synthesized with solid-phase temperature-assisted synthesis methodology. While eDNA is well‐recognised as one of the prime factors in the establishment of P. aeruginosa biofilms [39, 43], it has also been demonstrated to have such a role in other biofilm‐forming bacteria [50, 51].

eDNA initiates biofilm formation by binding with bacterial extracellular bio‐molecules such as polysaccharides, peptides/enzymes. Antimicrobial concentrations necessary to inhibit bacterial biofilms can be up to times higher than those needed to inhibit the same bacteria grown planktonically.

Thus, in the presence of therapeutically available antibiotic concentrations biofilms remain viable after treatment. The formation of biofilms on biomaterials causes biofilm-associated infections.

Available treatments often fail to fight the microorganisms in the biofilm, creating serious risks for patient well-being and life. Due to their significant antibiofilm activities, antimicrobial peptides are being intensively investigated in this regard.

A promising approach is a combination therapy that aims to. surfaces. Mixed-species of biofilms exist in most environments, but single-species biofilms last in a variety of pathogenic infections and on the surface of therapeutic embeds [20].

Biofilms of single-species are vitally important in the current area of research. Biofilm forming Pseudomonas aeruginosa is the most premeditated single. @article{osti_, title = {Mechanism of action and in vitro activity of short hybrid antimicrobial peptide PV3 against Pseudomonas aeruginosa}, author = {Memariani, Hamed and Shahbazzadeh, Delavar and Sabatier, Jean-Marc and Memariani, Mojtaba and Karbalaeimahdi, Ali and Bagheri, Kamran Pooshang, E-mail: [email protected]}, abstractNote = {Antimicrobial peptides are attractive.

SPLUNC1 is a multifunctional protein of the airway with antimicrobial properties. We previously reported that it displayed antibiofilm activities against P. aeruginosa. The goal of this study was to determine whether (1) the antibiofilm property is broad (including S.

aureus, another prevalent organism in cystic fibrosis); (2) the α4 region is responsible for such activity; and (3), if so.Susceptibility of Pseudomonas aeruginosa biofilm to α-helical peptides: D-enantiomer of LL Front.

Microbiol. 2, ().Crossref, Medline, CAS, Google Scholar; De La Fuente-Nunez C, Korolik V, Bains M et al. Inhibition of bacterial biofilm formation and swarming motility by a small synthetic cationic peptide. Antimicrob. Transcriptome Analysis of Pseudomonas aeruginosa Growth: Comparison of Gene Expression in Planktonic Cultures and Developing and Mature Biofilms Journal of Bacteriology, Vol.No.

18 Bacterial colonization on different suture materials—A .